The Tenth q-bio Summer School - New Mexico: Membrane Biology

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About the Membrane Biology focus area

Lead organizer: Mara Steinkamp (E-mail)

Faculty

Bridget Wilson, Ph.D. University of New Mexico

Diane Lidke, Ph.D. University of New Mexico

Keith Lidke, Ph.D. University of New Mexico

Topics

Membrane dynamics

The impact of spatial organization on signaling at the membrane

Fluorescence microscopy: Single Particle Tracking and Superresolution Imaging

Hands-On Workshops

Single Particle Tracking of the IgE Receptor on Live Cells: Acquisition and Analysis

Super-resolution Imaging and Analysis of Receptor Clustering

Software

Please install the following software to prepare for scheduled computer labs:

MATLAB (MathWorks, Natick, MA) - license available courtesy of MathWorks if required (contact organizers for details)

Recommended Reading

Single Particle Tracking: Freeman, S.A., V. Jaumouille, K. Choi, B.E. Hsu, H.S. Wong, L. Abraham, M.L. Graves, D. Coombs, C.D. Roskelley, R. Das, S. Grinstein, and M.R. Gold. 2015. Toll-like receptor ligands sensitize B-cell receptor signalling by reducing actin-dependent spatial confinement of the receptor. Nat Commun. 6:6168. doi: 10.1038/ncomms7168.

Pryor, Meghan M., Shalini T. Low-Nam, Ádám M. Halász, Diane S. Lidke, Bridget S. Wilson, and Jeremy S. Edwards. 2013. Dynamic Transition States of ErbB1 Phosphorylation Predicted by Spatial Stochastic Modeling. Biophysical Journal. 105:1533-1543. doi:10.1016/j.bpj.2013.07.056

McCabe Pryor, M., M.P. Steinkamp, A.M. Halasz, Y. Chen, S. Yang, M.S. Smith, G. Zahoransky-Kohalmi, M. Swift, X.-P. Xu, D. Hanein, N. Volkmann, D.S. Lidke, J.S. Edwards, and B.S. Wilson, Orchestration of erbb3 signaling through heterointeractions and homointeractions. Molecular Biology of the Cell, 2015;26:4109-4123.

Super-Resolution: Nan, X., T.M. Tamgüney, E.A. Collisson, L.-J. Lin, C. Pitt, J. Galeas, S. Lewis, J.W. Gray, F. McCormick, and S. Chu. 2015. Ras-GTP dimers activate the Mitogen-Activated Protein Kinase (MAPK) pathway. Proceedings of the National Academy of Sciences. 112:7996-8001.