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The Ninth q-bio Summer School - Albuquerque: Membrane biology

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About the Membrane Biology focus area

Lead scientific organizer: Mara Steinkamp

Faculty

Topics

  • Fluorescence microscopy
  • Cellular Potts modeling of cell-cell interactions and tissues
  • Membrane dynamics
  • T cell mobility

Hands-On Workshops

Single Particle Tracking of the IgE Receptor on Live Cells: Acquisition and Analysis

Super-resolution Imaging and Analysis of Receptor Clustering

Software

Please install the following software to prepare for scheduled computer labs:

  • CompuCell3D
  • MATLAB (MathWorks, Natick, MA) - license available courtesy of MathWorks if required (contact organizers for details)

Recommended Reading

Single Particle Tracking

  • Freeman, S.A., V. Jaumouille, K. Choi, B.E. Hsu, H.S. Wong, L. Abraham, M.L. Graves, D. Coombs, C.D. Roskelley, R. Das, S. Grinstein, and M.R. Gold. 2015. Toll-like receptor ligands sensitize B-cell receptor signalling by reducing actin-dependent spatial confinement of the receptor. Nat Commun. 6:6168. doi: 10.1038/ncomms7168.
  • Pryor, Meghan M., Shalini T. Low-Nam, Ádám M. Halász, Diane S. Lidke, Bridget S. Wilson, and Jeremy S. Edwards. 2013. Dynamic Transition States of ErbB1 Phosphorylation Predicted by Spatial Stochastic Modeling. Biophysical Journal. 105:1533-1543. doi:10.1016/j.bpj.2013.07.056

Super-Resolution

  • Nan, X., T.M. Tamgüney, E.A. Collisson, L.-J. Lin, C. Pitt, J. Galeas, S. Lewis, J.W. Gray, F. McCormick, and S. Chu. 2015. Ras-GTP dimers activate the Mitogen-Activated Protein Kinase (MAPK) pathway. Proceedings of the National Academy of Sciences. 112:7996-8001.

CompuCell3D

  • Andasari, V., R.T. Roper, M.H. Swat, and M.A. Chaplain. 2012. Integrating intracellular dynamics using CompuCell3D and Bionetsolver: applications to multiscale modelling of cancer cell growth and invasion. PLoS One. 7:e33726.
  • Swat, M.H., G.L. Thomas, A. Shirinifard, S.G. Clendenon, and J.A. Glazier. 2015. Emergent Stratification in Solid Tumors Selects for Reduced Cohesion of Tumor Cells: A Multi-Cell, Virtual-Tissue Model of Tumor Evolution Using CompuCell3D. PLoS ONE. 10:e0127972.