In recent years, it has become clear that pre-mRNA 3’ end processing is a dynamic process, with different transcript forms being generated from a given gene in different tissues and different cell states. Moreover, highly proliferative states such as cancer systematically favour cleavage and polyadenylation at 5' proximal sites, which modulates the malignancy of the cells. Our group has previously uncovered that the level of cleavage factor I (CF Im), a core component of 3’ end processing machinery, strongly influences the choice of polyadenylation sites. To further understand the mechanisms underlying this regulatory effect, we constructed a genome-wide catalogue of polyadenylation sites and used computational analyses that shed light on the impact of CF Im and other factors of the machinery.
