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Live Cell Imaging Methods to Quantify Signaling Molecule Dynamics
From Q-bio
Nicholas L. Andrews, Krishnan Radhakrishnan, Stanly Steinberg, Keith A. Lidke, Janet Pfeiffer, Thomas M. Jovin, Philippe Lenormand, Bridget S. Wilson, Janet M. Oliver, and Diane S. Lidke
- Abstract
- Examining protein distributions and dynamics in live cells using high-resolution bio-imaging methods and biophysical techniques can reveal information about the real-time activities of cell signaling networks. Modeling the temporal changes that can be observed with fluorescence microscopy and their relationship to protein function along complex signaling pathways can help to shed light on the underlying mechanisms that regulate cell signaling. Here, we describe the use of quantum dots (QDs) and Visible Fluorescent Protein (VFP) labels to track and model the dynamics of membrane receptors and cytoplasmic signaling proteins.
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